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. 2021 Jan 7;16(8):1660–1670. doi: 10.4103/1673-5374.303045

Figure 3.

Figure 3

Effects of exendin-4 and DA-CH5 on 6-OHDA-induced ROS production in SH-SY5Y cells.

Cells were exposed to 350 µM 6-OHDA with or without 100 nM of exendin-4 or DA-CH5 for 24 hours. (A) ROS generation in SH-SY5Y cells was detected using flow cytometry. (B) Quantitative results of intracellular ROS levels (fluorescence intensity of 2′,7′-dichlorofluorescin diacetate). Data are expressed as the mean ± SD of quadruplicate independent experiments. *P < 0.001, vs. control group (CON); †††P < 0.001, vs. 6-OHDA group; §P < 0.05, vs. 6-OHDA + exendin-4 group (one-way analysis of variance followed by Tukey’s multiple comparison test). 6-OHDA: 6-Hydroxydopamine; DCFH-DA: 2′,7′-dichlorofluorescin diacetate, a fluorescent probe; exendin-4 and DA-CH5: glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist; PD: Parkinson’s disease; ROS: reactive oxygen species.