Additional Table 1.
Repeated measurement design analysis of variance of mRNA expression levels of BDNF and its-related enzymes and receptors in the cortex and hippocampus after hypoxia-ischemia
| Variables | DF | SS | MS | F | P |
|---|---|---|---|---|---|
| BDNF in cortex | |||||
| Intervene | 1 | 55.786 | 55.786 | 1357.966 | <0.001 |
| Intergroup error | 12 | 0.493 | 0.041 | ||
| Time | 2.961 | 37.208 | 12.567 | 67.914 | <0.001 |
| Time × Intervene | 2.961 | 37.550 | 12.682 | 68.538 | <0.001 |
| Repeated measurement error | 35.531 | 6.574 | 0.185 | ||
| BDNF in hippocampus | |||||
| Intervene | 1 | 105.018 | 105.018 | 408.284 | <0.001 |
| Intergroup error | 12 | 3.087 | 0.257 | ||
| Time | 2.056 | 177.547 | 86.363 | 128.322 | <0.001 |
| Time × Intervene | 2.056 | 71.447 | 34.754 | 51.638 | <0.001 |
| Repeated measurement error | 24.670 | 16.603 | .673 | ||
| Furin in cortex | |||||
| Intervene | 1 | 3.715 | 3.715 | 75.125 | <0.001 |
| Intergroup error | 12 | 0.593 | 0.049 | ||
| Time | 1.585 | 15.022 | 9.480 | 61.647 | <0.001 |
| Time × Intervene | 1.585 | 11.761 | 7.422 | 48.265 | <0.001 |
| Repeated measurement error | 19.015 | 2.924 | 0.154 | ||
| Furin in hippocampus | |||||
| Intervene | 1 | 4.518 | 4.518 | 25.177 | <0.001 |
| Intergroup error | 12 | 2.153 | 0.179 | ||
| Time | 2.120 | 75.233 | 35.484 | 186.055 | <0.001 |
| Time × Intervene | 2.120 | 34.712 | 16.372 | 85.844 | <0.001 |
| Repeated measurement error | 25.442 | 4.852 | 0.191 | ||
| MMP-9 in cortex | |||||
| Intervene | 1 | 161366.853 | 161366.853 | 216.546 | <0.001 |
| Intergroup error | 12 | 8942.235 | 745.186 | ||
| Time | 1.001 | 836216.193 | 835450.050 | 223.008 | <0.001 |
| Time × Intervene | 1.001 | 849030.666 | 848252.782 | 226.425 | <0.001 |
| Repeated measurement error | 12.011 | 44996.629 | 3746.284 | ||
| MMP-9 in hippocampus | |||||
| Intervene | 1 | 1.947 | 1.947 | 59.656 | <0.001 |
| Intergroup error | 12 | 0.392 | 0.033 | ||
| Time | 3.046 | 11.729 | 3.851 | 143.417 | <0.001 |
| Time × Intervene | 3.046 | 13.792 | 4.528 | 168.641 | <0.001 |
| Repeated measurement error | 36.553 | 0.981 | 0.027 | ||
| PAI-1 in cortex | |||||
| Intervene | 1 | 11.457 | 11.457 | 242.113 | <0.001 |
| Intergroup error | 12 | 0.568 | 0.047 | ||
| Time | 1.349 | 22.308 | 16.535 | 84.569 | <0.001 |
| Time × Intervene | 1.586 | 21.615 | 13.632 | 81.940 | <0.001 |
| Repeated measurement error | 16.190 | 3.165 | 0.196 | ||
| PAI-1 in hippocampus | |||||
| Intervene | 1 | 2.854 | 2.854 | 26.247 | <0.001 |
| Intergroup error | 12 | 1.305 | 0.109 | ||
| Time | 1.795 | 250.344 | 139.495 | 286.018 | <0.001 |
| Time × Intervene | 1.795 | 107.396 | 59.842 | 122.699 | <0.001 |
| Repeated measurement error | 21.536 | 10.503 | 0.488 | ||
| Sortilin in cortex | |||||
| Intervene | 1 | 0.81 | 0.810 | 23.975 | <0.001 |
| Intergroup error | 12 | 0.405 | 0.034 | ||
| Time | 1.925 | 15.732 | 8.172 | 101.121 | <0.001 |
| Time × Intervene | 1.925 | 16.371 | 8.504 | 105.228 | <0.001 |
| Repeated measurement error | 23.101 | 1.867 | 0.081 | ||
| Sortilin in hippocampus | |||||
| Intervene | 1 | 15.721 | 15.721 | 49.677 | <0.001 |
| Intergroup error | 12 | 3.798 | 0.316 | ||
| Time | 1.169 | 333.997 | 285.594 | 193.870 | <0.001 |
| Time × Intervene | 1.169 | 182.769 | 156.282 | 106.089 | <0.001 |
| Repeated measurement error | 14.034 | 20.673 | 1.473 | ||
| TPA in cortex | |||||
| Intervene | 1 | 12.793 | 12.793 | 204.557 | <0.001 |
| Intergroup error | 12 | 0.75 | 0.063 | ||
| Time | 1.577 | 31.791 | 20.153 | 107.549 | <0.001 |
| Time × Intervene | 1.577 | 31.82 | 20.172 | <0.001 | |
| Repeated measurement error | 18.93 | 3.547 | 0.187 | ||
| tPA in hippocampus | |||||
| Intervene | 1 | 26.223 | 26.223 | 57.468 | <0.001 |
| Intergroup error | 12 | 5.476 | 0.456 | ||
| Time | 1.406 | 244.098 | 173.587 | 90.627 | <0.001 |
| Time × Intervene | 1.406 | 117.113 | 83.283 | 43.481 | <0.001 |
| Repeated measurement error | 16.874 | 32.321 | 1.915 | ||
| TrkB in cortex | |||||
| Intervene | 1 | 8.998 | 8.998 | 428.852 | <0.001 |
| Intergroup error | 12 | 0.252 | 0.021 | ||
| Time | 1.96 | 27.859 | 14.213 | 227.932 | <0.001 |
| Time × Intervene | 1.96 | 25.224 | 12.869 | 206.374 | <0.001 |
| Repeated measurement error | 23.521 | 1.467 | 0.062 | ||
| TrkB in hippocampus | |||||
| Intervene | 1 | 0.677 | 0.677 | 5.503 | 0.037 |
| Intergroup error | 12 | 1.476 | 0.123 | ||
| Time | 1.337 | 67.601 | 50.556 | 147.812 | <0.001 |
| Time × Intervene | 1.337 | 27.225 | 20.361 | 59.529 | <0.001 |
| Repeated measurement error | 16.046 | 5.488 | 0.342 |
BDNF: Brain-derived neurotrophic factor; DF: degree of freedom; MMP-9: matrix metallopeptidase 9; MS: mean square; PAI-1: plasminogen activator inhibitor 1; SS: sum of squares; tPA: plasminogen activator, tissue type.