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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Immunol. 2021 Apr 15;206(9):2135–2145. doi: 10.4049/jimmunol.2000899

Figure 1. S5A mice express equivalent levels of LPL without evidence of S5 phosphorylation.

Figure 1.

A. Schematic of LPL with three identified phosphorylation sites (S5, S7 and T89) in the regulatory N-terminal domain indicated. ABD = actin-binding domain. B. Schematic indicating that “S5A” represents endogenous LPL locus in which S5 is converted to non-phosphorylatable alanine residue. C. Immunoblot of whole spleen lysates from WT, S5A and LPL−/− mice. Anti-actin immunoblot indicates equivalent protein loading. Anti-LPL immunoblot indicates equivalent expression of endogenous LPL in S5A mice. Specific anti-S5 phosphorylation antibody indicates complete ablation of S5 phosphorylation of native LPL in S5A mice at baseline. D. Confocal microscopy of F-actin morphology of residential peritoneal macrophages from WT and S5A mice. F-actin illuminated using phalloidin (green), talin illuminated using anti-talin mAb (red), and nuclei using DAPI (blue). Arrows indicate clusters of podosomes. Scale bar = 6 μm. Images representative of two independent experiments.