Figure 4. Reduction in MZM splenic population in S5A mice revealed by immunofluorescence.

A. Schematic illustrating three tissue-resident lineages of splenic macrophages. B. Immunofluorescence of frozen splenic sections derived from naïve WT, S5A, “B6” and LPL^−/− mice. Splenic lineage markers are labeled with anti-CD209b (MZM), anti-CD162 (RPM) and anti-CD169 (MMM; red) and nuclei are counter-stained with DAPI (blue). Scale bars indicate 100 μm. WP = white pulp, RP = red pulp.