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. 2021 Jul 29;7(4):e602. doi: 10.1212/NXG.0000000000000602

Figure 1. Yeast Complementation Studies With Human ZDHHC15 Variants.

Figure 1

(A) Human ZDHHC15 protein depicting transmembrane, DHHC-CRD domains and the position of missense variants detected in individual cases. (B) Representative images of FM-464 vacuolar membrane staining in pfa3 yeast strain transformed with empty vector (pfa3), yeast PFA3 (PFA3), or human ZDHHC15 (ZDHHC15) after 2-hour incubation with 2 mM DTT. (C) Quantification of the number of cells with more than 6 vacuoles after incubation with DTT. ***Indicates significance determined by 2-tailed t test p < 0.0001 compared to ZDHHC15 control (grey bar). DTT = Dithiothreitol.