Fig. 4. Auxiliary mediated NCL to generate natively-linked ubiquitinated peptides and proteins. (A) Photocleavable auxiliary- mediated NCL to generate ubiquitinated H2B peptide. (B) 2-(aminooxy)ethanethiol-mediated chemical ubiquitination. (C) 1-(2,4-Dimethoxyphenyl)-2-mercaptoethyl-mediated chemical ubiquitination.
