Summary of pharmacologically active compounds from Gou Teng detected in 20 V DART-MS analysis of powdered product and extracts and corresponding reported biological activity of the compounds. X designates compounds detected in all samples, while B indicates the compound was only detected in products purchased in Beijing, or NY for products purchased in New York. * designates M − OH adduct. Bolded compounds are reported main pharmacologically active compounds.

Gou Teng
#	Compound	Biological activity	Adduct	Adduct mass	Extraction method
					Powder	H2O	EtOH	MAE	EtOH : CHCl3	Acid wash
1*	Catechin	Antioxidant26	–OH	273.0763			X
	Epicatechin	Antioxidant26	–OH
1	Catechin	Antioxidant26	+H	291.0869	X	X	X	X
	Epicatechin	Antioxidant26	+H
2	Akuammigine	Weak antagonist of pre & postsynaptic α-adrenoceptors27	+H	353.1865	X	X	X	X	X
	Tetrahydroalstonine	Antagonist of pre α2-adrenoceptors27	+H
3	Hirsuteine	5-HT3 antagonist28	+H	367.2022	X	X	X	X	X
4	Isopteropodine	Modulate function of G protein-coupled muscarinic M(1) acetylcholine & 5-HT(2) receptors29	+H	369.1814	NY	NY	NY	NY	NY
	Mitraphylline	Anti-inflammatory30	+H
	Pteropodine	Modulate function of G protein-coupled muscarinic M(1) acetylcholine & 5-HT(2) receptors29	+H
5	Dihydrocorynantheine	Vasodilator5	+H	369.2178	B	B	B	B	B
	Hirsutine	5-HT3 antagonist28	+H
6	Corynoxeine	5-HT3 antagonist28	+H	383.1971	X	X	X	X	X
	Isocorynoxeine	5-HT3 antagonist28	+H
7*	Campesterol	Inhibits cholesterol absorption31	–OH	383.3678			X		X	X
8	Corynoxine B	Inhibitor of central dopamine release32	+H	385.2127	X	X	X	X	X
	Isorhynchophylline	5-HT3 antagonist,28 antagonist of NMDA-type ionotropic glutamate receptor33	+H
	Rhynchophylline	5-HT3 antagonist (Nakamura), antagonist of NMDA-type ionotropic glutamate receptor32	+H
9*	Stigmasterol	Inhibits cholesterol absorption31	–OH	395.3678	X		X	X	X
10*	β-Sitosterol	Inhibits cholesterol absorption31	–OH	397.3834	X	X	X	X	X	X
9	Stigmasterol	Inhibits cholesterol absorption31	+H	413.3783	X		X		X	X
11*	Ursolic acid	Anti-inflammatory, Antihyperlipidemic34	–OH	439.3576	X	X	X	X	X	X
11	Ursolic acid		+H	457.3682	X		X		X	X