REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PARP	Cell Signaling Technology	Cat# 9542; RRID:AB_2160739
Celaved Caspase-3 (D175)	Cell Signaling Technology	Cat# 9661; RRID:AB_2341188
eIF4E	BD	Cat# 610270; RRID:AB_397665
p-eIF4E Ser209	Cell Signaling Technology	Cat# 9721; RRID:AB_561095
beta-Actin	Sigma-Aldrich	Cat# A5316; RRID:AB_476743
Pmpcb	Protein Tech	Cat# 16064-1-AP; RRID:AB_2167122
Tfeb	Bethyl Laboratories	Cat# A303-673A; RRID:AB_11204751
a-Tubulin	Sigma-Aldrich	Cat# T8203; RRID:AB_1841230
LC3A/B	Cell Signaling Technology	Cat# 4108; RRID:AB_2137703
c-Myc (D84C12)	Cell Signaling Technology	Cat# 5605; RRID:AB_1903938
Ras	Cell Signaling Technology	Cat# 3965; RRID:AB_2180216
Atf4 (D4B8)	Cell Signaling Technology	Cat# 11815; RRID:AB_2616025
Xbp-1	Abcam	Cat# Ab37152; RRID:AB_778939
Magoh	Protein Tech	Cat# 12347-1-AP; RRID:AB_2265988
eIF4AIII/eIF4A3	Bethyl Laboratories	Cat# A302-981A; RRID:AB_10748369
Rbm8a	Bethyl Laboratories	A301-033A; RRID:AB_2300943
Bcl-xL (H5)	Santa Cruz	Cat# sc-8392; RRID:AB_626739
Bacterial and virus strains
MegaX DH10B T1R Electrocomp Cells	Thermo Fisher Scientific	C640003
Chemicals, peptides, and recombinant proteins
A-1331852	MedChemExpress	HY-19741
eFT508	eFFECTOR Therapeutics
Trizol	Sigma-Aldrich	T9424-200ML
Trizol LS	Invitrogen	10296010
Matrigel	Corning	CB40234
PhosSTOP	Roche	4906837001
Complete Mini proteosome inhibitors	Roche	11836170001
Glycogen	Roche	10901393001
Paraformaldehyde
Poly-lysine	Sigma	P4707-50ML
Critical commercial assays
FITC-Annexin V	Thermo Fisher Scientific	A13199
UPRmt TF Activation Profiling	Signosis	FA-1010
Cell Titer Glo	Promega	G9241
Polybrene	Millipore Sigma	TR-1003-G
Lipofectamine 2000	Thermo Fisher Scientific	11662019
Lipofectamine LTX	Thermo Fisher Scientific	15338100
TOPO-TA	Invitrogen	450641
Super Signal West Pico Plus Chemiluminescent substrate	Thermo Scientific	34580
NE-PER Nuclear and Cytoplasmic Extraction Reagents	Thermo Scientific	78835
Direct-zol RNA Microprep	Zymo Research	R2061
High-Capacity cDNA Reverse transcription kit	Applied Biosystems	4368814
PowerUP SYBR Green master mix	Applied Biosystems	A25741
TurboDNA-free kit	Invitrogen	AM1907
Dual-luciferase Reporter Assay System	Promega	E1910
SuperScript III first-strand synthesis kit	Invitrogen	18080044
Prolong Diamond Antifade mountant with DAPI	Invitrogen	P36971
Ribo-Zero	Illumina	20040526
ScriptSeq v2 RNA-seq library preparation kit	Epicenter	SSV21124
Deposited data
RNA seq Data	This paper	GEO: GSE175417
Experimental models: Cell lines
MEFs eIF4E+/−	This paper
Oligonucleotides
See Table S3	This paper
Recombinant DNA
pBabe-Neo-LargeT	Hahn et al., 2002	Addgene #1780
pCL-Eco	Naviaux et al., 1996	Addgene #12371
pX458	Ran et al., 2013	Addgene #48138
human HRASV12	Serrano et al., 1997	Addgene #18749
human c-Myc	Boehm et al., 2005	Addgene #10674
psPAX2	Didier Trono	Addgene #12260
pMD2.G	Didier Trono	Addgene #12259
pLKO.1	Stewart et al., 2003	Addgene #8453
pMSCV	Clontech	634401
pGL3	Promega	E1751
pRL	Promega	E2231
pLG15	Luke Gilbert
sgRNA expressing vectors	This paper
pMSCV-GFP-Tfeb	This paper
pMSCV-GFP-Pmpcb	This paper
TFEB 5’UTR containing pGL3 reporters	This paper
mouse CRIPSRi library expressing 5 sgRNAs for each gene	IGI CRISPR screening core at UCSF
Software and algorithms
ImageJ		https://imagej.nih.gov/ij/
Fiji		https://imagej.net/software/fiji
ImageLab software	BioRad
Prism	GraphPad	Version 9.1.0
R Studio		https://www.rstudio.com/
HISAT v2.0.5		http://daehwankimlab.github.io/hisat2/
RSEM v1.3.0		https://github.com/deweylab/RSEM/releases
DAVID v6.8		https://david.ncifcrf.gov/
‘survminer’ R package		https://github.com/kassambara/survminer
AdobePhotoshop	Adobe
Adobe Illustrator	Adobe