Table 2. Comparison of Zika viral titer between Plaque Forming Unit (PFU), Focus Forming Unit (FFU), real time RT-PCR (Ct) and droplet digital RT-PCR (copy number/ml).
Virus name | Cell-based | Molecular-based | ||
---|---|---|---|---|
PFU | FFU | Ct | Copy number/ml | |
MUMT-1/2016 | 5.60 x 107 | 2.40 x 107 | 13.214 | 9.47 x 108 |
MU-DMSC-2/2016 | 3.60 x 106 | 1.12 x 106 | 14.043 | 8.93 x 108 |
MU-DMSC-3/2016 | 1.04 x 106 | 5.53 x 105 | 13.416 | 9.61 x 108 |
MU-DMSC-4/2016 | 1.26 x 107 | 9.93 x 106 | 12.621 | 1.23 x 109 |
MU-DMSC-5/2016 | 1.17 x 106 | 1.40 x 106 | 13.208 | 9.70 x 108 |
MU-DMSC-6/2016 | 9.88 x 105 | 1.20 x 106 | 12.339 | 1.42 x 109 |
MU-DMSC-1/2017 | 1.83 x 107 | 1.26 x 107 | 13.660 | 5.99 x 108 |
MU-DMSC-2/2017 | 1.48 x 106 | 1.13 x 106 | 12.603 | 1.09 x 109 |
MU-DMSC-3/2017 | 4.00 x 106 | 1.53 x 106 | 13.247 | 9.66 x 108 |
MU-DMSC-4/2017 | 1.24 x 106 | 1.46 x 106 | 13.731 | 9.29 x 108 |
MU-DMSC-5/2017 | 1.00 x 106 | 1.03 x 106 | 14.311 | 4.99 x 108 |