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. Author manuscript; available in PMC: 2021 Jul 30.
Published in final edited form as: ACS Chem Biol. 2020 Dec 22;16(1):52–57. doi: 10.1021/acschembio.0c00935

Figure 2.

Figure 2.

Prodrug binding assays. (A) Luminescent output of HCT-116 receiver-cells with varying doses of either TMP or pNZ-TMP. TMP shows an EC50 of 5 nM while pNZ has significantly reduced affinity. (B) Dose response assay with TMP and pNZ-TMP using destabilized YFP as reporter protein. (C) Dose response assay with TMP and pNZ-TMP using destabilized IL-2 as reporter protein. (D) Time course assay of reporter protein stabilization in fluorescent receiver-cells, with either TMP or pNZ-TMP, shows differences in fluorescence as early as 3 hours. Each experiment was repeated 3 times. Data is from a representative experiment; error bars represent S.D. for n = 6, 3, 4, and 4 technical replicates, respectively (A, B, C, & D).