Extended Data Fig.7: Spearman-rank cross-correlation analysis of the representation of CAZymes, monosaccharides and glycosidic linkages in the faecal communities of participants consuming the pea fibre snack prototype.
a,b, Correlations between the log2 fold-change of statistically significant, HOSVD-defined discriminatory CAZyme gene abundances (matched by time and participant) to the log2 fold-change in levels of monosaccharides and glycosidic linkages at days 25 and 35 (fibre snack consumption), and days 45 and 49 (post-intervention phase) normalized to day 14 (pre-intervention phase; n=72 faecal samples analysed; 12 participants). Green boxes in panel a highlight a significant correlation between GH43_37 (arabinofuranosidase) and arabinose, a prominent monosaccharide component of pea fibre. Panel b provides evidence that participant microbiomes contain CAZymes that cleave multiple branches of pea fibre arabinan, resulting in accumulation of its 1,5-arabinofuranose backbone in faeces. See Supplementary Results for further details. *, P <0.05; **, P<0.01. Abbreviations: glucose (Glc), galacturonic acid (GalA), arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), rhamnose (Rha), fucose (Fuc), fructose (Fru), glucuronic acid (GlcA), N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), allose (All), ribose (Rib), hexose (Hex), deoxyhexose (dHex); terminal (T), pyranose (p), furanose (f), undefined linkage (X).