Figure 4.

H₂S exposure activated the PI3K/Akt signaling pathway

Activation of PI3K/Akt signaling pathways in the IC after H₂S exposure was measured by Western blotting analyses. A, Phosphorylation of AKT at both S474 and T308 was increased at the immediate and early response time points following H₂S exposure compared to the breathing air control group. Expression of PI3K was also upregulated at the immediate and early response times following H₂S exposure compared to the breathing air control group. Phosphorylation of PKA C at T197 was also increased at the immediate and early response times points H₂S exposure. Phosphorylation of p-70S6k at T389 was increased at the immediate response to H₂S exposure. B, band intensities of Western blotting in A were measured and visualized in graph view. Significant differences were determined by t-test with comparison to the breathing air control group and were indicated with *(p<0.05) or **(p<0.01).