Figure 1.

Effects on MCF-7 cell inhibition at different times and with different doses of TAM, celastrol, and their combination. (a–h) The effects on MCF-7 cell inhibition with different doses of TAM, celastrol, and their combination at 24 h, 48 h, and 72 h, respectively. (i) The IC50 values of celastrol and tamoxifen on MCF-7 cells were 1.77 and 7.87 μM at 48 h, respectively. The IC50 value of tamoxifen was 4.79 μM when combined with celxastrol (0.3 μM). The empty dot below the line indicates a synergistic effect by isobolographic analysis.