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Effect of HIF-1α RNAi combined with ASP on HCC growth and angiogenesis in a SK-Hep1 xenograft nude mouse model. (a)-(b), (d) Tumor volume and weight. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus LV-shHIF1α + ASP group. (c), (e) Protein levels of HIF-1α, VEGF, AKT, p-AKT, ERK, and p-ERK assessed using western blot. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus control group. #P < 0.05, ##P < 0.01, and ###P < 0.001 versus LV-shHIF1α + ASP group.
