Table 2.
Percentage of recovery success and recovery efficiency of BCoV in three independent experiments. Each experiment was performed with a different sample in duplicate. 3.2 × 107, 1.3 × 107 and 5.7 × 106 genomic copies were inoculated in experiment 1, 2 and 3, respectively.
| BCoV Recovery efficiency | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| PEG precipitation |
SM flocculation |
Aluminum polychloride flocculation |
|||||||
| Sample | Concentrated sample# | % of recovery | Sample | Concentrated sample# | % of recovery | Sample | Concentrated sample# | % of recovery | |
| Experiment 1 | 1a | 48,333 | 0.2 | 1a | 1,159,500 | 3.6 | 1a | ND | ND |
| 1b | 67,500 | 0.2 | 1b | 150,167 | 0.5 | 1b | 500 | 0.002 | |
| 1a 1/10 | 858,333 | 2.6 | 1a 1/10 | 5,055,000 | 15.6 | 1a 1/10 | ND | ND | |
| 1b 1/10 | 1,641,667 | 5.1 | 1b 1/10 | 3,541,667 | 10.9 | 1b 1/10 | ND | ND | |
| Experiment 2 | 2a | 525,833 | 4.0 | 2a | ND | ND | 2a | ND | ND |
| 2b | 157,500 | 1.2 | 2b | ND | ND | 2b | ND | ND | |
| 2a 1/10 | 891,667 | 6.7 | 2a 1/10 | ND | ND | 2a 1/10 | ND | ND | |
| 2b 1/10 | 558,333 | 4.2 | 2b 1/10 | ND | ND | 2b 1/10 | ND | ND | |
| Experiment 3 | 3a | 250,833 | 4.4 | 3a | 2833 | 0.05 | 3a | ND | ND |
| 3b | 235,833 | 4.1 | 3b | 197,000 | 3.4 | 3b | ND | ND | |
| 3a 1/10 | 400,000 | 7.0 | 3a 1/10 | 3333 | 0.06 | 3a 1/10 | ND | ND | |
| 3b 1/10 | 291,667 | 5.1 | 3b 1/10 | 1,051,666 | 18.4 | 3b 1/10 | ND | ND | |
| Mean (±SD) | PEG | 214,305 ± 173,880 | 2.4 ± 2.0 | SM | 377,375 ± 527,939 | 1.9 ± 1.9 | Al | NA | NA |
| PEG 1/10 | 773,611 ± 488,433 | 5.1 ± 1.6 | SM 1/10 | 2,412,917 ± 2,303,170 | 8.9 ± 8.0 | Al 1/10 | NA | NA | |
| BCoV Recovery success* | Undiluted sample | 6/6(100 %) | 4/6(66 %) | 1/6(17 %) | |||||
| Diluted sample | 6/6(100 %) | 4/6(66 %) | 0/6 | ||||||
| Total | 12/12 (100 %) | 8/12(66 %) | 1/12(8 %) | ||||||
NA: Not applicable, ND: Not detected. SD: Standard deviation.
*
Positive qPCR reactions/total qPCR reactions (% positive samples).
#
genomic copies.