Figure 2.
Mechanically responsive TPCs (mrTPCs) are a subset of TPCs with high numbers of mitochondrially expressed genes (MEGs) that are enriched among normal TPCs
(A) GO term analysis of more highly expressed genes in the normal donor-derived TPC cohort. Darker red colors correspond to higher p values (shown). Genes were automatically chosen by the Enrichr program.
(B) UMAP plots showing MEG levels within the total TPC populations for normal and disease cohorts. Data represent three biological replicates per cohort, and both normal and diseased cohorts are shown separately.
(C) Histogram of MEG percentages for normal and disease cohorts. Data represent three biological replicates per cohort, and both normal and diseased cohorts are shown separately.
(D) Violin plot showing individual sample MEG percentage. Black dashed lines represent sample averages. ∗p < 0.05. Data represent individual biological replicates per sample.
(E) Histogram of MEG percentages for TPCs in either resting or post-stretching condition. Grey bars of the histogram are resting TPC MEG percentages, and yellow bars are after stretching TPCs. Data represent one biological replicate and one technical replicate of the single-cell profiling.
(F) Ridge plot showing MEG percentage for TPCs within each TPC subset. Data represent all six biological replicates from both cohorts.
(G) Individual sample association with mrTPC subsets. Each dot represents an individual biological replicate, and three biological replicates from each cohort are shown. Error bars represent standard deviation.
(H) Percentage of cells in healthy and disease cohorts that are apoptotic, dead, or both in the resting and stretched conditions. Blue bars represent the normal cohort, and red bars represent diseased cohort, with three biological replicates per cohort, and one technical replicate per cohort member. ∗p < 0.05. Error bars represent min/max values.
(I) Mitotracker red CMXRos staining. Dotted line represents 0. Counts are log10 of the ratio of the median stretched compared with its resting counterpart. Three biological replicates per cohort, and one technical replicate per biological sample.
(J) qRT-PCR results for TFAM, TUFM, and BAX. Three biological replicates per cohort, and three technical replicates per cohort. ∗p < 0.05.