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. 2021 Jul 21;2(7):100343. doi: 10.1016/j.xcrm.2021.100343
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

TotalSeq-A0251 anti-human Hashtag 1 Antibody BioLegend 394601; RRID:AB_2750015
TotalSeq-A0252 anti-human Hashtag 2 Antibody BioLegend 394603; RRID:AB_2750016
TotalSeq-A0254 anti-human Hashtag 4 Antibody BioLegend 394607; RRID:AB_2750018
APC/Cyanine7 anti-human CD45 Antibody BioLegend 368515; RRID:AB_2566375
PerCP/Cyanine5.5 anti-human CD31 Antibody BioLegend 303131; RRID:AB_2566174
FITC Mouse Anti-Human CD34 BD PharMingen 560942; RRID:AB_10562559
APC Mouse anti-Human CD73 BD PharMingen 560847; RRID:AB_10612019
PE-Cy7 Mouse Anti-Human CD90 BD PharMingen 561558; RRID:AB_10714644
PE Mouse anti-Human CD105 BD PharMingen 560839; RRID:AB_2033932
FITC Annexin V Apoptosis Detection Kit with PI BioLegend 640914
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl Antibody BioLegend 400128; RRID:AB_2892538
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl Antibody BioLegend 400149; RRID:AB_893680
PE-Cy7 Mouse IgG1 κ Isotype Control BD PharMingen 557872; RRID:AB_396914
APC Mouse IgG1 κ Isotype Control BD PharMingen 554681; RRID:AB_398576
PE Mouse IgG1, κ Isotype Control BD PharMingen 555749; RRID:AB_396091
FITC Mouse IgG1, κ Isotype Control BD PharMingen 555748; RRID:AB_396090

Biological samples

Human tendon progenitor cells Patellar tendon samples attained by Dr. Jason Dragoo N/A

Chemicals, peptides, and recombinant proteins

Fixable Viability Stain 450 BD PharMingen 562247

Deposited data

Single cell RNA sequencing datasets This paper GEO accession GEO: GSE150482

Oligonucleotides

TUFM qPCR primers IDT Hs.PT.58.4007037
TFAM qPCR primers IDT Hs.PT.58.4391001
BAX qPCR primers IDT Hs.PT.56a.19141193.g
GAPDH qPCR F primer IDT CAATGACCCCTTCATTGACC
GAPDH qPCR R primer IDT TTGATTTTGGAGGGATCTCG

Software and algorithms

R (4.0.1) CRAN N/A
Seurat Stuart∗,Butler∗,et al., Cell 2019 N/A
Excel Microsoft N/A
GraphPad Prism GraphPad N/A
CITE-seq-Count https://github.com/Hoohm/CITE-seq-Count N/A
MAST Finak et al., Genome Biol 2015 N/A
FlowJo Tree Star N/A