Antibodies |
|
TotalSeq-A0251 anti-human Hashtag 1 Antibody |
BioLegend |
394601; RRID:AB_2750015
|
TotalSeq-A0252 anti-human Hashtag 2 Antibody |
BioLegend |
394603; RRID:AB_2750016
|
TotalSeq-A0254 anti-human Hashtag 4 Antibody |
BioLegend |
394607; RRID:AB_2750018
|
APC/Cyanine7 anti-human CD45 Antibody |
BioLegend |
368515; RRID:AB_2566375
|
PerCP/Cyanine5.5 anti-human CD31 Antibody |
BioLegend |
303131; RRID:AB_2566174
|
FITC Mouse Anti-Human CD34 |
BD PharMingen |
560942; RRID:AB_10562559
|
APC Mouse anti-Human CD73 |
BD PharMingen |
560847; RRID:AB_10612019
|
PE-Cy7 Mouse Anti-Human CD90 |
BD PharMingen |
561558; RRID:AB_10714644
|
PE Mouse anti-Human CD105 |
BD PharMingen |
560839; RRID:AB_2033932
|
FITC Annexin V Apoptosis Detection Kit with PI |
BioLegend |
640914 |
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl Antibody |
BioLegend |
400128; RRID:AB_2892538
|
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl Antibody |
BioLegend |
400149; RRID:AB_893680
|
PE-Cy7 Mouse IgG1 κ Isotype Control |
BD PharMingen |
557872; RRID:AB_396914
|
APC Mouse IgG1 κ Isotype Control |
BD PharMingen |
554681; RRID:AB_398576
|
PE Mouse IgG1, κ Isotype Control |
BD PharMingen |
555749; RRID:AB_396091
|
FITC Mouse IgG1, κ Isotype Control |
BD PharMingen |
555748; RRID:AB_396090
|
|
Biological samples |
|
Human tendon progenitor cells |
Patellar tendon samples attained by Dr. Jason Dragoo |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
Fixable Viability Stain 450 |
BD PharMingen |
562247 |
|
Deposited data |
|
Single cell RNA sequencing datasets |
This paper |
GEO accession GEO: GSE150482
|
|
Oligonucleotides |
|
TUFM qPCR primers |
IDT |
Hs.PT.58.4007037 |
TFAM qPCR primers |
IDT |
Hs.PT.58.4391001 |
BAX qPCR primers |
IDT |
Hs.PT.56a.19141193.g |
GAPDH qPCR F primer |
IDT |
CAATGACCCCTTCATTGACC |
GAPDH qPCR R primer |
IDT |
TTGATTTTGGAGGGATCTCG |
|
Software and algorithms |
|
R (4.0.1) |
CRAN |
N/A |
Seurat |
Stuart∗,Butler∗,et al., Cell 2019 |
N/A |
Excel |
Microsoft |
N/A |
GraphPad Prism |
GraphPad |
N/A |
CITE-seq-Count |
https://github.com/Hoohm/CITE-seq-Count |
N/A |
MAST |
Finak et al., Genome Biol 2015 |
N/A |
FlowJo |
Tree Star |
N/A |