Figure 5.

Neurite outgrowth is reduced in HSN1 iPSCdSNs and is not stimulated with neurotrophic factors

(A and B) Photomicrographs of 6-month-old (A) control and (B) HSN1 neurons 24 h after dissociation and replating, and immunocytochemically stained for NF200.

(C–E) Quantification of various neurite outgrowth parameters including (C) total neurite length, ∗p = 0.0013 by 2-way ANOVA (F = 23.11, df = 1), (D) the longest single branch, ∗p = 0.0002 by 2-way ANOVA (F = 40.11, df = 1), and (E) the number of branch points, ∗p = 0.035 by 2-way ANOVA (F = 6.46, df = 1).

(F) Quantification of neurite length 24 h after replating treated with (+NT) or without (−NT) neurotrophic factors, ∗p = < 0.0001, by one-way ANOVA (F = 160.8, df = 3) with Tukey’s post hoc test for individual comparisons (∗p = < 0.0001 for control −NT versus control +NT).

(G) Photomicrographs of 6-month-old control and HSN1 neurons 24 hours after replating, being treated with and without neurotrophic factors, and immunocytochemically stained for NF200.

Colors on graphs represent different iPSC lines and all error bars are SEM.