ETEC challenge affects cell viability and inflammatory gene expression in IPEC-J2 cells. IPEC-J2 cells (1 × 105 cells/well) were treated with E. coli K88 at different final concentrations (1 × 105, 1 × 106, 1 × 107, and 1 × 108 CFU/well) for 3 h. A, cell viability; B, the expression of IL-1β; C, the expression of TNF-α. Data are presented as mean ± SEM. a–bValues within a column differ if they do not share a common superscript (P < 0.05)