Fig. 2.

Batch cultures of E. coli BL21(DE3)pLysS (pBAD/3C/bTGase) in TB media. At 2 h, the culture temperature was reduced to 20 °C, the pro-bTGase gene was induced by the addition of 0.4 mM IPTG, and cultivations proceeded for 20 h; gene 3C protease was induced by the addition of 0.2% L-arabinose, followed by 6 h of cultivation. A (■) biomass DCW (g/L); (△) glucose (g/L); (▽) acetate (g/L). The vertical lines mark the time of induction of pro-bTGase and 3C protease. B Cell plasmid stability test: (★) cell count in LB medium containing 34 μg/mL chloramphenicol; (✰) cell count in LB medium containing 100 μg/mL carbenicillin and 34 μg/mL chloramphenicol. All experimental runs were performed in duplicates