Table 1.
Kinetic parameters for mTGase production in different media in 30 h of induction. Results are the mean of duplicates
| Cultivation strategy (culture medium) |
Dry cell weight | Specific activity | Volumetric activity | Enzymatic productivity |
|---|---|---|---|---|
| (g/L) | (U/mgprotein) | (U/L) | Pp (U/L·h) | |
|
Batch (TB) |
6.13 ± 0.10 | 3.12 ± 0.08 | 19.17 ± 0.81 | 0.64 ± 0.03 |
|
Fed-batch (TB then glucose) |
17.56 ± 0.96 | 6.43 ± 0.66 | 19.17 ± 3.21 | 0.64 ± 0.11 |
|
Fed-batch (M9) |
17.07 ± 3.44 | 9.14 ± 0.42 | 29.38 ± 1.54 | 0.98 ± 0.05 |
PP, enzymatic productivity