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. 2021 Jul 30;4:928. doi: 10.1038/s42003-021-02427-0

Fig. 3. Intraperitoneal acetate injection increases serum acetate levels and primes neutrophils in mice.

Fig. 3

a Mice received an intra-peritoneal acetate (Ac) injection (500 mg/kg) followed by analysis of blood acetate concentrations (isolated from the tail vein) and neutrophil phenotypes 30 min and 6 h after injection. b Acetate (Ac) i.p. injection caused a two-fold serum acetate increase 30 min after injection and normal serum acetate levels were restored after 6 h. c Neutrophils isolated from mice treated with acetate showed enhanced complement (CD11b) and Fcγ (CD16/CD32) receptor expression compared to PBS-treated mice. d In vivo acetate (Ac) injection caused increased ROS production upon stimulation with phorbol-12-myristat-13-acetate (PMA). Data in all panels represent geometric means from six animals. *P < 0.05; **P < 0.01 significant difference versus the indicated condition as calculated by Mann–Whitney U test.