Table 1.

Validation of DAPs using parallel reaction monitoring (PRM) analysis.

Protein Accession	Protein name	Protein gene	G/B Ratio (PRM)*	G/B P value (PRM)	G/B Ratio (TMT)*	G/B P value (TMT)
A0A452EPH7	Phosphoglycerate mutase	BPGM	3.54	1.84E−02	1.64	0.0078
I1W1N4	Ras-related protein Rab-11B	RAB11B	4.28	5.70E−03	1.73	0.0033
A0A452FSK9	ATP-citrate synthase	ACLY	4.40	3.88E−02	1.52	0.0277
A0A452E5B4	Peroxiredoxin	PRDX5	2.88	3.19E−02	1.53	0.0345
A0A452DLM2	Spermadhesin-1	LOC102175091	2.84	8.60E−02	1.75	0.0221
A0A452EVX4	T-complex protein 1 subunit alpha	TCP1	2.57	3.16E−02	1.59	0.0116
A0A452DSU1	Programmed cell death protein 5	PDCD5	1.86	2.08E−01	1.53	0.0480
A0A452G6T9	Testis tissue sperm-binding protein Li 69n	PSMD14	3.72	6.53E−03	1.65	0.0002
A0A452FJ29	Ubiquitin-like modifier-activating enzyme 1	UBA1	2.78	1.23E−02	1.50	0.0005
A0A452EC76	Zonadhesin	ZAN	0.01	3.27E−03	0.35	0.0000
A0A452E294	Superoxide dismutase	SOD2	0.24	1.00E−03	0.41	0.0001
A0A452EFP3	Sperm equatorial segment protein 1	SPESP1	0.05	8.31E−05	0.51	0.0001
A0A452DL42	Thioredoxin reductase 2, mitochondrial	TXNRD2	0.24	1.48E−02	0.57	0.0001
A0A452E876	Acrosin-binding protein	ACRBP	0.19	2.95E−02	0.41	0.0009
A0A452DWD6	Zona pellucida binding protein	ZPBP	0.23	7.26E−03	0.38	0.0000
A0A452E0C3	Heat shock protein family E (Hsp10) member 1	HSPE1	0.60	7.50E−02	0.66	0.0079
A0A452E1R9	Peroxiredoxin 1	PRDX1	0.39	1.60E−01	0.61	0.0470
A0A452DNC1	Aquaporin 7	AQP7	0.22	4.31E−06	0.60	0.0001
A0A452EAI1	Izumo sperm-egg fusion protein 4	IZUMO4	0.08	6.55E−03	0.44	0.0036

“B” represented the group with low motility; “G” represented the group with high motility.