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. 2021 Jul 30;12(8):754. doi: 10.1038/s41419-021-04041-8

Fig. 1. Severe ischemia/reperfusion-induced acute kidney injury (AKI) led to renal maladaptive repair with chronic inflammation and fibrosis.

Fig. 1

A Serum creatinine and B urea nitrogen levels followed IRI (n = 6 mice). The serum creatinine and urea nitrogen levels on day 0 represent normal baseline measured before surgery. #p < 0.001 defined as significant to baseline. C Representative images of HE staining on Sham, IRI-14d, and IRI-28d kidneys. D Representative images of F4/80 immunohistochemistry staining on Sham, IRI-14d, and IRI-28d kidneys. E Representative images of Sirius Red staining on Sham, IRI-14d, and IRI-28d kidneys. F Renal expression of α-SMA in Sham, IRI-14d, and IRI-28d mice. Shown are representative blots from at least three separate experiments with similar results. G Quantification of F4/80-positive cells in kidney (counted). (n = 3 for Sham, and n = 6, 6 for IRI-14d and IRI-28d, respectively). H Semi-quantification of Sirius Red staining. (n = 3 for Sham, and n = 6, 6 for IRI-14d and IRI-28d, respectively). Scale bar = 50 µm in all images of C, D, and E. All values are means ± SEM. **p < 0.01 and #p < 0.001 are defined as significant.