Antibodies |
|
anti-VACht antibody |
Synaptic Systems |
Cat. No. 139103; RRID: AB_887864
|
anti-TH antibody |
Millipore |
Cat. No. AB1542; RRID:AB_90755
|
|
Critical commercial assays |
|
SuperScript VILO Master Mix |
Thermo Fisher Scientific |
Cat#:11756500
|
|
Deposited data |
|
Genotype-Tissue Expression (GTEx) Portal |
GTEx Analyis V8 |
dbGaP Accession phs000424.v8.p2; https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000424.v8.p2
|
Single-nucleus RNA sequencing analysis of adult mouse cerebral cortex |
Hu et al.2017 |
GSE106678 |
Single cell data from the human CNS |
Allen Brain Map |
RRID:SCR_017001; https://portal.brain-map.org/atlases-and-data/rnaseq/human-multiple-cortical-areas-smart-seq
|
RNAseq comparisons to the peripheral nervous system |
Zeisel et al.2018 |
http://mousebrain.org/genesearch.html; Mousebrain.org.level1/L1_Enteric.loom; Mousebrain.org.level1/L1_Sympathetic.loom |
RNAseq comparisons to the peripheral nervous system |
Furlan et al.2016 |
http://linnarssonlab.org/sympathetic/ |
Raw sequencing data generated in this study |
GEO database |
GSE154119; GEO SuperSeries GSE154411
|
Raw and processed HT-qPCR data of single neurons from the pig RAGP |
GEO database |
GSE149212; GEO SuperSeries GSE154411
|
Acquisition of single neurons and regional neuronal samples from the porcine Right Atrial Ganglionic Plexus (RAGP) through Laser Capture Miscrodissection |
Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science |
https://doi.org/10.26275/56h4-ypua |
Spatially Tracked Single-Cell-Scale RNAseq of Porcine Right Atrial Ganglionic Plexus (RAGP) Neurons |
Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science |
https://doi.org/10.26275/kabb-mkvu |
Transcriptional Diversity of Single Neurons in the Porcine Right Atrial Ganglionic Plexus (RAGP) |
Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science |
https://doi.org/10.26275/5jki-b4er |
Spatially Tracked Single-Neuron Transcriptomics of a Female Porcine Right Atrial Ganglionic Plexus (RAGP) |
Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science |
https://doi.org/10.26275/qkzi-b1mq |
Spatially Tracked Single-Neuron Transcriptomics of a Male Porcine Right Atrial Ganglionic Plexus (RAGP) |
Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science |
https://doi.org/10.26275/255m-00nj |
Dataset containing figures from the paper as well as code and necessary files to generate them. |
Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science |
https://doi.org/10.26275/jdws-d7md |
|
Experimental models: organisms/strains |
|
Model organism: Yucatan minipig |
S&S Farms |
N/A |
|
Software and algorithms |
|
R version 3.6.3 (Holding the Windsock) |
The R Foundation for Statistical Computing |
https://www.r-project.org/ |
Subread R package |
Liao et al., 2019 |
https://bioconductor.org/packages/release/bioc/html/Rsubread.html |
ComBat-seq |
Zhang et al., 2020 |
sva package; https://bioconductor.org/packages/release/bioc/html/sva.html
|
DESeq2 |
Love et al., 2014 |
https://bioconductor.org/packages/release/bioc/html/DESeq.html |
SCnorm |
Bacher et al., 2017 |
https://bioconductor.org/packages/release/bioc/html/SCnorm.html |
Real-Time PCR Analysis Software |
Fluidigm |
https://www.fluidigm.com/software |
NormqPCR |
Bioconductor |
RRID:SCR_003388; https://bioconductor.org/packages/release/bioc/html/NormqPCR.html
|
cluster R package |
Comprehensive R Archive Network (CRAN) |
https://cran.r-project.org/web/packages/cluster/index.html |