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. 2021 Jul 19;24(7):102713. doi: 10.1016/j.isci.2021.102713
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

anti-VACht antibody Synaptic Systems Cat. No. 139103; RRID: AB_887864
anti-TH antibody Millipore Cat. No. AB1542; RRID:AB_90755

Critical commercial assays

SuperScript VILO Master Mix Thermo Fisher Scientific Cat#:11756500

Deposited data

Genotype-Tissue Expression (GTEx) Portal GTEx Analyis V8 dbGaP Accession phs000424.v8.p2; https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000424.v8.p2
Single-nucleus RNA sequencing analysis of adult mouse cerebral cortex Hu et al.2017 GSE106678
Single cell data from the human CNS Allen Brain Map RRID:SCR_017001; https://portal.brain-map.org/atlases-and-data/rnaseq/human-multiple-cortical-areas-smart-seq
RNAseq comparisons to the peripheral nervous system Zeisel et al.2018 http://mousebrain.org/genesearch.html; Mousebrain.org.level1/L1_Enteric.loom; Mousebrain.org.level1/L1_Sympathetic.loom
RNAseq comparisons to the peripheral nervous system Furlan et al.2016 http://linnarssonlab.org/sympathetic/
Raw sequencing data generated in this study GEO database GSE154119; GEO SuperSeries GSE154411
Raw and processed HT-qPCR data of single neurons from the pig RAGP GEO database GSE149212; GEO SuperSeries GSE154411
Acquisition of single neurons and regional neuronal samples from the porcine Right Atrial Ganglionic Plexus (RAGP) through Laser Capture Miscrodissection Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science https://doi.org/10.26275/56h4-ypua
Spatially Tracked Single-Cell-Scale RNAseq of Porcine Right Atrial Ganglionic Plexus (RAGP) Neurons Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science https://doi.org/10.26275/kabb-mkvu
Transcriptional Diversity of Single Neurons in the Porcine Right Atrial Ganglionic Plexus (RAGP) Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science https://doi.org/10.26275/5jki-b4er
Spatially Tracked Single-Neuron Transcriptomics of a Female Porcine Right Atrial Ganglionic Plexus (RAGP) Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science https://doi.org/10.26275/qkzi-b1mq
Spatially Tracked Single-Neuron Transcriptomics of a Male Porcine Right Atrial Ganglionic Plexus (RAGP) Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science https://doi.org/10.26275/255m-00nj
Dataset containing figures from the paper as well as code and necessary files to generate them. Stimulating Peripheral Activity to Relieve Conditions (SPARC) data portal, sparc.science https://doi.org/10.26275/jdws-d7md

Experimental models: organisms/strains

Model organism: Yucatan minipig S&S Farms N/A

Software and algorithms

R version 3.6.3 (Holding the Windsock) The R Foundation for Statistical Computing https://www.r-project.org/
Subread R package Liao et al., 2019 https://bioconductor.org/packages/release/bioc/html/Rsubread.html
ComBat-seq Zhang et al., 2020 sva package; https://bioconductor.org/packages/release/bioc/html/sva.html
DESeq2 Love et al., 2014 https://bioconductor.org/packages/release/bioc/html/DESeq.html
SCnorm Bacher et al., 2017 https://bioconductor.org/packages/release/bioc/html/SCnorm.html
Real-Time PCR Analysis Software Fluidigm https://www.fluidigm.com/software
NormqPCR Bioconductor RRID:SCR_003388; https://bioconductor.org/packages/release/bioc/html/NormqPCR.html
cluster R package Comprehensive R Archive Network (CRAN) https://cran.r-project.org/web/packages/cluster/index.html