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. 2021 Jul 30;11:15569. doi: 10.1038/s41598-021-95239-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The representative micrographs of the follicles from primary to antral stages, obtained from adult (6-week-old) and aged (52-week-old) mouse ovaries. The micrographs of the follicles were captured under a light microscope (Zeiss, Oberkochen, Germany). All the follicles displayed their characteristic features either in the adult or aged groups. Morphologically normal follicles at similar diameters were used in the subsequent experiments. The average diameter of each follicle type is provided below its own micrograph as mean ± standard deviation (SD). It should be noted that the micrographs of the primary and secondary follicles were captured under an original magnification of 200 ×, whereas those of the preantral and antral follicles were captured at an original magnification of 100 ×. Scale bars are equal to 50 µm. A antrum, AS antral space, O oocyte.