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. 2021 Jul 30;11:15569. doi: 10.1038/s41598-021-95239-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The telomerase activity in the ovarian follicles from primary to antral stages, obtained from adult (6-week-old) and aged (52-week-old) groups. For this analysis, we used primary (n = 34 and 21), secondary (n = 30 and 46), preantral (n = 30 and 25), and antral (n = 30 and 25) follicles collected from five adult and three aged mice, respectively. The TRAP assay results of the follicles from primary to antral stages in the adult (A) and aged (B) groups. As expected, internal control (IC) product was generated in all reaction tubes. An additional heat-treated control (abbreviated as heat) was run alongside each sample as telomerase is a heat-sensitive enzyme. In both adult and aged groups, we found that telomerase activity reached high levels in the preantral and antral follicles when compared to the primary and secondary follicles. It is worth noting that that this assay was technically repeated twice. NC negative control, PF primary follicles, SF secondary follicles, PrF preantral follicles, AF antral follicles, TSR8 control template, bp base pair.