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. 2021 Jul 30;12:4629. doi: 10.1038/s41467-021-24887-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A, B CryoFIB/SEM images of two sequential slices separated by 80 nm. Black arrows point to virus particles in a vesicle (V). Purple arrow points to small electron-dense material lining the outside of virus-containing vesicle. C Tomographic slice of cryoFIB lamella depicting SARS-CoV-2 assembly, with DMV portals (yellow arrow), assembling viruses (blue arrow), assembled virus (black arrow), viral spikes on SMV membranes (red arrows), transporting vesicles around the assembly site (pink arrow), and a nucleopore (black arrowhead). D Density segmentation of C, displaying three virus particles (black arrows) and two assembly sites (blue arrows). E An enlarged view (at a different angle) of boxed area in C, showing assembled virus (black arrow), assembling viruses (blue arrows), spikes (red arrows), and spike-containing vesicles (pink arrows). F Large intracellular virus-containing vesicle (LVCV) full of readily assembled viruses. G Subtomogram average of viral spikes of intracellular viruses from cell lamellae at 16 Å resolution, fitted with an atomic model of spike trimer (PDB 6VXX)¹⁶. Scale bar is 300 nm in A–C and 100 nm in E, F.