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. 2021 Jul 30;12:4634. doi: 10.1038/s41467-021-24900-4

Fig. 2. DimAβ AβOs bind to dendrites and postsynaptic spines but have no direct cytotoxic effect on primary mouse neurons.

Fig. 2

Primary mouse neurons (DIV15–22) were treated with 0.5 µM dimAβ AβOs or 1 µM Aβ40 for 3 and 24 h. a DimAβ AβOs localized to neuronal dendrites both after 3 and 24 h of treatment, where they partially co-localized with phalloidin, a marker for synaptic spines. Arrows indicate co-localization of dimAβ with phalloidin. Scale bar, 5 µm. The experiment was independently repeated four times with similar results. b Nuclei of primary neurons were stained with NucBlue and analyzed with respect to shape and size. Representative images of normal and dense nuclei. Scale bar, 10 µm. c Quantification of normal and dense nuclei of primary neurons after vehicle control, Aβ40, or dimAβ AβO treatment revealed no direct cytotoxicity. N = 3; around 300 nuclei were analyzed for each condition. Error bars represent SEM. Statistical analysis was done by two-way ANOVA with Tukey’s test for multiple comparisons and yielded no significant differences between the experimental groups.