Fig. 3. DimAβ AβOs induce pathological somatodendritic missorting of Tau.

Primary mouse neurons (DIV15–22) were treated with 0.5 µM dimAβ AβOs or 1 µM Aβ40 for 3 and 24 h. a Representative images of cell bodies of primary neurons after treatment with Aβ. Neurons were stained with anti-Tau (K9JA) antibody; nuclei were stained with NucBlue. DimAβ AβO-treated neurons show strong enrichment of fluorescence signal of Tau in the soma only after 24 h of treatment. Insets show magnification of white boxed areas in the somata. Scale bar, 10 µm. b Quantification of Tau enrichment in the soma of primary neurons. Fluorescence intensities of cell bodies were quantified and normalized to control-treated neurons after 3 h of treatment. N = 4, 30 cells were analyzed for each condition. Error bars represent SEM. Statistical analysis was done by two-way ANOVA with Tukey’s test for multiple comparisons. Statistical significance: ****p < 0.0001.