Fig. 5. Aβ42 and dimAβ AβOs accumulate in endosomes/lysosomes.

SH-SY5Y cells were treated with Aβ42 monomers (top row) or dimAβ AβOs (bottom row) and co-localization with endo-lysosomal compartments was analyzed. 1.1 µM Aβ42 (containing 9% HiLyte 647-labeled Aβ42, top row) or 1.1 µM dimAβ AβOs (in monomer equivalents, formed from a dimAβ solution containing 9% AbberiorStar 520SXP-labeled dimAβ, bottom row) were added to the cells. After 24 h, the medium was exchanged with fresh medium supplemented with 50 nM Yellow HCK-123 LysoTracker dye. Scale bar, 5 µm. N = 3, at least three images were acquired for each treatment to ensure reproducibility.