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. 2021 Jul 30;11:15511. doi: 10.1038/s41598-021-94798-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cross-sectional areas (CRA) and inner fluid reservoir areas (IFRA) analysis of WT nasospheroids from different healthy WT subjects. Spheres were analyzed by live-cell imaging after DMSO or FSK incubation. Dots and triangles show mean FR values up to 60 min. Bars represent SEM. Both CRA and IFRA results present significant differences between the DMSO and the FSK stimulus. T-test also showed statistically different results for CRA and IFRA (*p = 0.0316; paired t-test). Calculated AUC also showed significant differences between CRA and IFRA trajectories (****p < 0.0001). Similar results were observed after analysing slopes (results showing slopes of the same experiments are available in Supplementary Information). Results were a combination from spheres grown in conditions 1 and 2. Graphic created with GraphPad Prism version 6³³.