Fig. 2.
Representative gel pictures of PCR amplicons of toxic genes in Clostridioides difficile. A: Multiplex PCR for 16Sr DNA (1062 bp) tcdA (629 bp) tcdB (410 bp) ctdB (262 bp) and cdtA (221 bp) genes using the following primers; (PS13, PS14) - (F3345, R3989) – (F5670, R6079A, R6079B) - (F617, R878) - (F739A, F739B, R958), respectively. B: Single amplicon for PCR of tcdC (475 bp) gene by F (17), R (+462) primers.
