Fig. 2.

VCP mutant expression. (A) Schematic of VCP protein with the relative position of pathogenic mutations. *N384H was embryonic lethal and was not studied further. (B) Table of human and homologous Drosophila VCP mutations. (C) Representative western blot of VCP heterozygous mutant expression levels. (D) Quantification of total VCP protein abundance. (E) Quantification of wild-type versus mutant VCP protein levels. Data are mean±s.e.m. n=4 independent biological replicates. **P<0.01, ***P<0.001, ****P<0.0001 (one-way ANOVA with Dunnett's multiple comparisons).