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. 2021 Jul 12;14(7):dmm048603. doi: 10.1242/dmm.048603

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — Mitochondrial respiratory function in VCP mutants. (A) Schematic illustration of substrate coupling to mitochondrial respiratory pathways evaluated by high-resolution respirometry. (B-K) Acceptor control ratio (ACR) (B,C) and respiration supported by proline (D,E), succinate (F,G) and glycerol-3-phosphate (G3P) in the presence of ADP (P) (H-I) and FCCP (electron transfer) (J-K) in aged male or female wild type (WT) and VCP mutants. Data are mean±s.e.m. n=4 independent biological replicates with three flies/replicate. *P<0.05, **P<0.01, ***P<0.01 (one-way ANOVA with Dunnett's multiple comparisons). P, OXHPOS; ET, electron transfer.