Figure 2.

The RFLP and direct sequencing of SNPs in CYP1A1 rs4646903. (A) Lanes:1,2,3,4,5,9,and 10 showing two bands of 209 and 133 for TC genotype and confirmed for sample in lane 3 in the direct sequencing seen (black arrow) in picture (B). Three bands are present in lanes: 6,7 and 8 of 342,209 and 133bp for the CC genotype which is seen in the direct sequencing chromatogram(black arrow) in (C). Lane 11 is a homozygous variant (TT) of the SNP that is verified by direct sequencing in (D) which shows a single signal of thymine (black arrow). The last lane (12) is a control sample with no added restriction enzyme and has a single band of 342bp