Table 2.

Digestion Enzymes, Their Supplier, Digestion Conditions, the Size of the Digested Fragments in Base Pair (bp) and the Amino Acid Changes

The SNP	Restriction enzyme	Digestion conditions	Products size	Amino acid
"CYP1A1 rs1048943 ILe 462Val"	EcoT14I (StyI)	5µl (PCR product) 1µl (enzyme) 4µl (buffer) 1hour at 37°C	212bp (AA)	Isoluecine
"CYP1A1 rs4646903 "	(TaKaRal®/ Japan)	5µl(PCR product) 1µl (enzyme) 4µl (buffer) 1hour at 37°C	190,212bp (AG)	Isoleucine/Valine
"CYP1B1 rs1056836 Leu 432Val "	MspI (HapII , HpaII) (TaKaRal®/ Japan)	5µl (PCR product) 1µl (enzyme) 4µl (buffer) 1hour at 37°C	190 bp (GG)	Valine