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. Author manuscript; available in PMC: 2021 Jul 31.
Published in final edited form as: Science. 2019 Dec 19;366(6472):1531–1536. doi: 10.1126/science.aav4011

Fig. 1. Endog-deficiency increases cmtDNA and type-I IFN signaling.

Fig. 1.

(A and B) Quantification of cmtDNA (A) and total mtDNA (B) in WT and Endog−/− MEFs. (C and D) ISG expression levels (C) and heat map analysis of RNAseq data (D) in WT and Endog−/− MEFs. (E and F) ISG expression levels in WT and Endog−/− MEFs as well as two independently generated ρ0 MEFs (ρ0 1 and ρ0 2) were determined by immunoblotting (E) and RT-qPCR (F). (G) ISG expression was measured in WT and Endog−/− MEFs after treatment with Mito(M)-TEMPO (10 μM) for 48 h. All values are presented as the mean ± SEM of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.005; nd, not detected; ns, not significant.