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. 2021 Jul 23;118(30):e2102718118. doi: 10.1073/pnas.2102718118

Fig. 2.

Fig. 2.

EZH2 inhibition-mediated up-regulation of CXCL10 is necessary for NK cell migration. (A) Schematic overview of NK cell migration assays. (B) Average number of NK cells that migrated toward conditioned media collected from SK-HEP-1 and PLC/PRF/5 cells treated with EZH2 inhibitors GSK343 (3 µM) and GSK126 (2 µM) or DMSO for 72 h. (C) Average number of NK cells that migrated toward conditioned media collected from SK-HEP-1 and PLC/PRF5 cells treated with the EED inhibitor EED226 (5 µM) or DMSO for 72 h. (D) Average number of NK cells that migrated toward conditioned media collected from SK-HEP-1 and PLC/PRF/5 cells ectopically expressing CXCL10 or the vector control. (E) Average number of NK cells that migrated toward conditioned media collected from SK-HEP-1 and PLC/PRF/5 cells expressing CXCL10 shRNAs or NS shRNA control that were treated with either GSK343 (3 µM) or DMSO for 72 h. Data are presented as mean ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001.