Fig. 2.

Conservation of Var64 amino acids in land plants. (A) Phylogenetic tree built using KAI2/DLK/DDK/D14 sequences from land plants and rooted using KAI2 sequences from hornworts. Sequences within each of the three colored clades were investigated in more detail for their similarity with variant 64 at positions 153, 157, and 190. (B) KAI2 divergent sequences from parasitic Orobanchaceae. (C) Conserved KAI2 sequences from angiosperms. (D) Conserved D14 sequences from angiosperms. Colored boxes beside the clades indicate amino acid similarity to its counterpart in Var64 at positions 153 (blue), 157 (green), and 190 (purple).