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. 2021 Jul 31;9:165. doi: 10.1186/s40168-021-01114-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The performance of HumGut versions in comparison to the standard Kraken2 database and UHGG collection. a. The boxplot shows the distribution of unclassified reads for the 3,534 analyzed healthy reference metagenome samples. The dashed line represents the k-mer database sizes (right y-axis). Every database version includes standard human genome sequences, in addition to database-specific (sub)sets of bacteria and archaea, and the difference in size is only due to differences in the latter. b The classification of an additional 963 human gut metagenomes, not part of the reference set