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. 2021 Jul 30;20:150. doi: 10.1186/s12934-021-01638-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Bioreactor production of rh Bri2 BRICHOS. a Fed-batch cultivation (Culture #9). After 28 h, the oxygen demand suddenly decreased, and the feeding was initiated. b Estimation of expression level by SDS-PAGE. A sample from the cultivation 19 h and 21 h after induction was diluted 10-times. S1–S4 served as standards to estimate the concentration of NT*-Bri2 BRICHOS. S₁ 0.24 mg/mL; S₂ 0.48 mg/mL; S₃ 0.95 mg/mL; S₄ 1.9 mg/mL. c IMAC purification. L: cell lysate; D: pellet after centrifugation; E1: IMAC eluate; FT1: Flow-through round 1; E2: IMAC eluate after re-loading FT1; FT2: Flow-through round 2