Skip to main content
. 2021 Jul 19;118(30):e2100073118. doi: 10.1073/pnas.2100073118

Fig. 2.

Fig. 2.

Raf36 negatively regulates SnRK2-dependent ABA response phenotypes in Arabidopsis seedlings. (A) Abundance of Raf36 mRNA transcripts measured by qRT-PCR. Total RNA was extracted from 1-wk-old WT Col-0 Arabidopsis seedlings treated with 50 µM ABA for indicated periods. Bars indicate means ± SE (n = 3), and asterisks showed significant differences by Dunnett’s test between ΔCt (nontreated) and ΔCt (ABA-treated) (*P < 0.05, **P < 0.01). (B) Dwarf phenotype of raf36 plants under normal condition. WT (Col-0), raf36-1, and raf36-2 plants grown at 22 °C under 16/8 h photoperiod for 29 d. (Scale bar, 3 cm.) (C and D) Quantification of the cotyledon greening rates of WT (Col-0), raf36-1, and raf36-2 on germination agar medium (GM) with or without 0.5 µM ABA. Data are means ± SE (n = 3). Each replicate contains 36 seeds. Photographs were taken 6 d after vernalization. (E and F) Quantification of the cotyledon greening rates of WT (Col-0), raf36-1, srk2dsrk2e, and raf36-1srk2dsrk2e on GM agar medium in the presence or absence of 0.5 µM ABA. Data are means ± SE (n = 3). Each replicate contains 36 seeds. Photographs were taken 6 d after vernalization.