Fig. 5.

Cross-reactivity studies of LEAD using other coronaviruses and noncoronavirus strains. Baseline-corrected SWVs recorded at optimized experimental conditions before (black lines) and after incubation of the electrode with (A) SARS-CoV-2, (B) SARS-CoV-2 UK variant B, and possible interfering viruses such as (C) H₁N₁-A/California/2009, (D) Influenza-B/Colorado, (E) herpes simplex virus-2, and (F) MHV. The following conditions were used for all experiment: frequency of 80 Hz, amplitude of 70 mV, and step potential 8 mV. All experiments were performed in 5.0 mmol⋅L⁻¹ [Fe(CN)₆]^−3/−4 in 0.1 mol⋅L⁻¹ KCl. The specificity studies were carried out using the following viral strains: MHV at 10⁸ PFU⋅mL⁻¹ (coronavirus); H₁N₁, A/California/2009; Influenza B, B/Colorado; HSV2, and herpes simplex virus-2 (all at 10⁵ PFU⋅mL⁻¹). The analysis of the contagious SARS-CoV-2 B.1.1.7 UK variant showed that the variant presented higher interaction between SP and ACE2 compared to SARS-CoV-2. Each virus was incubated in a final volume of 50 µL for 5 min. All viruses were in stored VTM and experiments were performed at room temperature.