Fig. 1.
XL-MS and biophysical studies reveal an interaction network in the Notch1–Jagged1 complex. (A) Notch1fe, Jagged1fe, and targeted sites are expressed in HEK293 cells and purified by IMAC and SEC. (B) Identification of regions in proximity in the Notch1fe–Jagged1fe complex by XL-MS using PhoX (44). (C) The purified full ectodomain samples and shorter regions of interest are used in quantitative binding experiments to confirm direct interactions, and in SAXS studies. (D) The resulting data provide insights into the molecular architecture of the Notch1–Jagged1 complex, represented here as a schematic in a cis setting.