Table 2.

Structural parameters derived from SAXS experiments

	Concentration (µM)	Rg (Å) Guinier	sRg range used in Guinier for Rg	Rg (Å) P(r)	Dmax (Å)	I0 (cm−1)
Notch1fe SEC-SAXS	n/a	105 ± 0.2	0.62–1.26	113	380	0.047 ± 5.7 × 10−4
Jagged1fe,HA SEC-SAXS	n/a	74.1 ± 0.6	0.44–1.29	74.3	240	0.07 ± 4.4 × 10−4
Jagged1fe,HA Batch	42	102 ± 0.4	0.49–1.15	110	430	0.26 ± 5.8 × 10−4
	21	96.4 ± 0.7	0.49–1.08	103	430	0.23 ± 8.7 × 10−4
	11	89.2 ± 1.0	0.49–1.10	90.2	330	0.19 ± 1.1 × 10−3
	5.3	81.2 ± 0.8	0.45–1.25	85.3	300	0.16 ± 1.2 × 10−3
Jagged1EGF8–11 Batch	230	31.7 ± 0.1	0.62–1.12	32.7	120	0.044 ± 6.4 × 10−5
	115	31.3 ± 0.1	0.69–1.22	32.7	115	0.045 ± 8.4 × 10−5
	58	31.5 ± 0.1	0.56–1.26	32.8	115	0.046 ± 1.0 × 10−4
	29	32.7 ± 0.4	0.64–1.16	32.5	110	0.047 ± 3.0 × 10−4
Jagged1CRD Batch	167	24.1 ± 0.0	0.40–1.09	24.3	90	0.036 ± 2.6 × 10−5
	83	23.3 ± 0.0	0.18–1.16	23.3	82	0.036 ± 3.0 × 10−5
	42	22.6 ± 0.1	0.21–1.29	22.8	78	0.036 ± 4.4 × 10−5
	21	22.7 ± 0.1	0.21–1.30	22.8	75	0.035 ± 7.5 × 10−5

SAXS batch data I₀ have been normalized by the sample concentration to allow for the comparison between samples. Nonnormalized I₀ values are available on SASBDB under the accession codes defined in Data Availability. n/a, not applicable.