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Spectroscopic characterization of recombinant BlsK. (A) Color and UV-visible spectrum analysis of BlsK protein. The blue curve represents BlsK protein and red curve is the buffer control. (B) EPR analysis of BlsK with (black) and without (blue) addition of 1 mM sodium dithionite. EPR parameters: microwave frequency, 9.39 GHz; microwave power, 2 mW; modulation amplitude, 3 Gauss; modulation frequency, 100 kHz; and temperature, 10 K. The protein concentration used was 10 mg /mL.
