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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Biomaterials. 2021 May 18;275:120898. doi: 10.1016/j.biomaterials.2021.120898

Figure 3. Developmental trajectories of tissues grown from an intermediate initial cell seeding density (n = 1166).

Figure 3.

a-c, Diffusion maps showing the trifurcating development of tissues. Color-coding denotes thickness ratio, GATA3+ cell ratio and culture time as indicated. d, Bifurcating trajectory of asymmetric embryonic-like tissues and mesodermal-like tissues. Color-coding denotes trunk and branch points. e-g, NANOG+ cell ratio, GATA3+ cell intensity and tissue thickness ratio as a function of pseudotime of tissue development. h, Bifurcating trajectory of amniotic-like tissues and mesodermal-like tissues. i-k, T+ cell intensity, GATA3+ cell ratio and tissue area as a function of pseudotime of tissue development. l, Intermediate states between asymmetric and amniotic branches. m-o, GATA3+ cell number, NANOG+ cell ratio and T+ cell intensity as a function of pseudotime of tissue development.