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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Biomaterials. 2021 May 18;275:120898. doi: 10.1016/j.biomaterials.2021.120898

Figure 5. Properties and developmental trajectories of tissues grown from a small initial cell seeding density (n = 555).

Figure 5.

a, Schematics of bifurcating tissue development. b, Tissue area and lumen area as a function of culture time. c, Total cell number and T+ cell number as a function of culture time. d, Percentages of three types of tissues over time. e, Tissue thickness ratio plotted against tissue equivalent diameter. f, T+ cell intensity plotted against tissue thickness ratio. Color-coding in e and f denotes culture time. g, Diffusion maps of tissues. Color-coding denotes tissue thickness ratio, GATA3+ cell ratio and culture time as indicated. h, Bifurcating trajectory of amniotic-like and asymmetric tissues. i, GATA3+ cell ratio, NANOG+ cell ratio, T+ cell ratio and thickness ratio as a function of psuedotime of tissue development.