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. 2021 Jul 31;38(9):105. doi: 10.1007/s12032-021-01554-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — EV characterization by NanoFCM and TEM. a Total particle concentrations by cell number at moment of CCM harvest demonstrate number of secreted EVs per million cells. Mean + SD were calculated of three replicates. b TEM images confirm the presence of negative-stained EVs, seen as cup-shaped vesicles in widefield view. Black scale bar represents 500 nm. White arrows indicate small EVs. c close-up TEM images of kidney EVs. White scale bar represents 100 nm. White arrows indicate small EVs